Device

Part:BBa_K1377000

Designed by: Jade Minseob Yoo   Group: iGEM14_Korea_U_Seoul   (2014-10-09)


promoter of pili A gene cluster + spaA front

Pilin A gene cluster is coding for pili A of Corynebacterium diphtheriae. It has four coding regions and one regulatory region. Regulatory region is its own promoter. Four coding regions are sortase A, enzyme that makes pili A and pilin spa A & B & C that are components of pili A . WIth these all regions, we can make pili A.

And we are going to modifying this part to generate "Our Favorite Protein Whip", especially spa A

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 109
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 838
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 109
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 109
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 1091
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 572


[edit]
Categories
Parameters
None